DONATE
FOR PHYSICIANS
HEALTH AND NUTRITION
ETHICAL RESEARCH & EDUCATION
MEDIA CENTER
LEGISLATIVE FOCUS
CLINICAL RESEARCH
EDUCATIONAL LITERATURE
MEMBERSHIP
SHOP

Connect with Us

 

 

The Physicians Committee



2014nutrition-matching


NEWS RELEASE January 23, 2007

Cruelty-Free Insulin Test Explained in New Journal Article

Testing for Insulin Without the Pitter-Patter of Little Feet; New Journal Article Outlines Mouse-Free and Animal-Serum-Free Method of Producing Cruelt

WASHINGTON—In the January issue of Clinical Biochemistry, scientists and physicians with the Physicians Committee for Responsible Medicine outline the method used to develop a cruelty-free ELISA for human insulin that uses monoclonal antibodies produced by cells cultured in an animal-serum-free medium. PCRM’s insulin ELISA, the first of its kind, provides precision and reliability equal to methods currently used in clinical research. PCRM’s insulin ELISA is now commercially available for research purposes from Millipore of St. Charles, Mo., which reports that it is selling briskly. The new ELISA also serves as a guide for the development of serum-free immunoassays for other hormones and bio-markers.

The ELISA, or enzyme-linked immunosorbant assay, is a method of detecting specific proteins in complex protein mixtures—in this case, for detecting insulin in human blood. Laboratories traditionally detect human insulin using antibodies produced by cells that had been placed into the abdomens of living mice. The procedure, called the ascites method, is so cruel that it is banned in some European countries. Even when the antibodies are produced from cells in test tubes, fetal bovine serum is commonly used to grow live cells. The serum is obtained from bovine fetuses by puncturing their hearts with a needle without the use of anesthesia.

PCRM’s insulin ELISA does not rely on the ascites method or fetal bovine serum. In addition to ethical advantages, growing cells without animal serum ensures that fewer variables are introduced into experiments, meaning that results are easily reproducible by different laboratories. The method outlined in Clinical Biochemistry, the official journal of the Canadian Society of Clinical Chemists, permits the growth of antibody-producing cells in a medium free of animal serum, enabling scientists to make cell culture safer and more humane.

“The ethical and scientific advantages of avoiding fetal calf serum and the ascites method are clear, and we hope the methodology used to produce our ELISA serves as a guide for developing other animal-serum-free immunoassays,” says Chad Sandusky, Ph.D., a co-author of the paper and PCRM’s director of research and toxicology.

Founded in 1985, the Physicians Committee for Responsible Medicine is a nonprofit health organization that promotes preventive medicine, conducts clinical research, and encourages higher standards for ethics and effectiveness in research.



Media Contact:
Patrick Sullivan
510-834-8680
psullivan@pcrm.org

Mail Options:
Subscribe to PCRM’s news release distribution list

Unsubscribe from PCRM’s news release distribution list


2014 Archive

2013 Archive

2012 Archive

2011 Archive

2010 Archive

2009 Archive

2008 Archive

2007 Archive

2006 Archive

2005 Archive

2004 Archive

2003 Archive

2002 Archive

2001 Archive

2000 Archive

 
This site does not provide medical or legal advice. This Web site is for informational purposes only.
Full Disclaimer | Privacy Policy

The Physicians Committee
5100 Wisconsin Ave., N.W., Ste.400, Washington DC, 20016
Phone: 202-686-2210     Email: pcrm@pcrm.org